Disable ads (and more) with a membership for a one time $4.99 payment
Why are biological indicators for the autoclave used?
Indicate that the correct temperature has been reached
Indicate that sterility has been achieved
Indicate a problem with the autoclave
Indicate that the correct pressure has been reached
The correct answer is: Indicate that sterility has been achieved
Biological indicators are used in the autoclave process primarily to confirm that sterility has been achieved. These indicators typically contain viable spores of a highly resistant microorganism, such as Geobacillus stearothermophilus, which are placed inside the autoclave load. After the sterilization cycle is complete, the indicators are taken out and cultured to determine if any growth has occurred. If the spores have been successfully killed, this indicates that the sterilization conditions—such as temperature, time, and steam penetration—were sufficient to achieve sterility. While other indicators might assess specific conditions like temperature or pressure, biological indicators focus specifically on the final outcome of the sterilization process—whether or not all microorganisms, including resistant spores, have been eradicated. This crucially assures that the items sterilized are safe for use in medical and laboratory settings.